Immunoglobulin E (IgE) ELISA kit promotion - Database & Sql Blog Articles

Test - lowercase jpg

Kaixin micro test

Test probe P100-M3

1206 ball warm white

Immunoglobulin E (IgE) ELISA Kit

Basic Information

Immunoglobulin E (IgE) is a class of antibody with a δ chain and is a major antibody involved in the regulation of pathogenesis such as allergic rhinitis, allergic asthma and eczema. Since the discovery of IgE by Japanese scholar Ishizaka in 1966, IgE research has made significant progress, and IgE receptors have been found on the surface of mast cells, basophils, eosinophils and macrophages, and various allergies. Disease patients, including allergic asthma patients, have isolated specific IgE for a variety of pollen, dust mites, molds and animal skin. In recent years, many cytokines such as IL-4 and γ-interferon have been involved in the regulation of IgE synthesis. IgE antibodies can initiate both rapid-onset allergic reactions and delayed-onset allergic reactions.

Immunoglobulin E (IgE) ELISA kit method of operation

1. Dilute the antibody to 1-10 ug/ml with buffer. 0.1 ml was added to the reaction well at 4 ° C overnight. The next day, the solution in the well was discarded and washed 3 times with washing buffer.

2. Loading: Add 0.05 ml of the sample to be tested to the above-mentioned coated reaction well, and incubate at 37 ° C for 1 hour. Then wash (both blank wells, negative control wells and positive control wells).

3. Add the enzyme-labeled antibody: Add freshly diluted enzyme-labeled antibody (diluted by titration) to 0.05 ml in each reaction well. Incubate at 37 ° C for 0.5 to 1 hour and wash.

4. Add substrate liquid to develop color: Add 0.1 ml of temporarily prepared TMB substrate solution to each reaction well at 37 ° C for 10 to 30 minutes.

5. Stop the reaction: add 2M sulfuric acid 0.05ml to each reaction well.

6. Judgment of results: The results can be observed directly on the white background with the naked eye: the darker the color in the reaction well, the stronger the positive degree, the negative reaction is colorless or very light, according to the depth of the color, with "+", The "-" sign indicates. The OD value can also be measured: on the ELISA detector, at 450 nm (if the color is developed by ABTS, 410 nm), the OD value of each well is measured after zero adjustment of the blank control well, if it is greater than 2.1 times the OD value of the specified negative control. Is positive.

The advantages of our elisa kit: simple, fast, high sensitivity, specificity, cost-effective, and low sample requirements

1. The operation is simple and fast. All the steps, there are maps, so that you can be simple and clear, no need to delay the time for research steps.

2. High sensitivity. The enzyme is an organic catalyst, and a small amount of enzyme can induce a large amount of catalytic reaction, resulting in an observation color reaction phenomenon. Our kits quantify them at ug, ng, and pg levels.

3. Strong specificity. Specificity is derived from the selectivity of the antibody or antigen. The use of internationally renowned imported antibody antigens guarantees the quality of the kit.

4. High cost performance and stable quality.

5. The amount of sample required is small.

If you need to know the details of this product, please feel free to contact us.