Basic conditions for the correct cultivation of cells - Shanghai Jinma Bio-华强电子网

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Cell culture refers to a culture technique in which cells are taken out from tissues in vivo to mimic the in vivo environment, to grow and reproduce, and to maintain their structure and function. The culture of the cell culture may be a single cell or a cell population.

1. Suitable cell culture medium

A suitable cell culture medium is one of the most important conditions for cell growth and proliferation in vitro. The medium not only provides cell nutrition and a basic substance for promoting cell growth and proliferation, but also provides a living environment for culturing cell growth and reproduction.

2, high quality serum

Currently, most synthetic media require the addition of serum. Serum is one of the most important components in cell culture fluids, and contains various growth factors and other nutrients required for cell growth.

3. Sterile non-toxic cell culture environment

Sterile and non-toxic operating environment and culture environment are the primary conditions for ensuring the success of cells in vitro. Cells cultured in vitro, due to lack of defense against microorganisms and toxic substances, may be caused by cell poisoning death once they are contaminated by microorganisms or toxic substances, or their own metabolic substances accumulate. Therefore, when cells are cultured in vitro, the cell survival environment must be kept sterile and non-toxic, and the cell metabolites should be removed in time.

4, constant cell growth temperature

To maintain the vigorous growth of cultured cells, there must be a constant and appropriate temperature.

5. Suitable gas environment

Gas is one of the necessary conditions for mammalian cell culture to survive. The required gases are mainly oxygen and carbon dioxide.

Shanghai Jinma Bio-Agent sells a variety of biological products such as cells and culture media. Welcome to inquire!