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F380J106MMA original authentic, spot stock brand NICHICON
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Electronic scale crystal oscillator 3.2*2.5mm 3225 16M (16.000MHZ) 12PF 10PPM 20PPM 30PPM
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Probe domestic double-head probe 038-BB-5.7L The shape of the two ends of the needle is outside the pointed needle
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Huaqiang Square crystal original spot stock
How to properly resuscitate the cells and freeze them, what should you pay attention to? What's wrong? Just remember the time and take it out. So, what are the concerns in the process of cell resuscitation? What is the role of cell viability and morphological examination? Not to mention it, please see below: Vitality check – Never use unhealthy cells, there may be pollution (fungi , Mycoplasma, etc., if any contamination is found without hesitation! Morphological examination – check the intrinsic morphology and growth behavior of the cells. Ok, start cutting into the frozen cells: add a new medium – add a new medium the day before you start cryopreserving the cells. Harvest cells when the cells grow to 70% monolayer, count the number of viable cells, adjust the cell density to ~5 x 106 cells/ml (according to different cell types) with cryopreservation solution - wash cells with cryopreservation solution and freeze Resuspend the cells in a resuscitation solution, and have different types of cryopreservation solutions. Select the most suitable cryopreservation solution according to the cell type (commonly used cryopreservation components): – 5-10% DMSO – Take care to ensure that DMSO does not contain other toxic substances. – 5-15% Glycerol – If cells are grown in serum-free medium, they should be frozen and resuscitated in 50% conditioned medium (cells are grown in serum-free medium for 24 hours). Mark the cell type, date, frozen deposit and other information on the cryotube, and ensure that each frozen tube does not exceed 1.5ml. Program cooling is the key to ensure the activity of frozen cells, using Mr. Frosty The device) guarantees a cooling rate of 1°-3°C and is placed in a refrigerator at -80°C overnight. If there is no Mr. Frosty device, you can use the foam box, cotton, etc. that are common in the laboratory (original is a laboratory diaper, huh, huh, I don't know what bird it is.) Record the number and location of the frozen tubes before placing them in the liquid nitrogen tank. Transfer the cryotube to the liquid nitrogen tank at the fastest speed. Therefore, it is best to use dry ice in this step or immerse the cryotube in a small box containing liquid nitrogen. Also note that there is not enough space on the cryotube to record the details of the cells. It is very important to keep a record! There is one more important thing, you must keep the same one in a liquid nitrogen tank in a different place. Cells, so as not to have a problem with one of the liquid nitrogen tanks! Cell resuscitation - the correct way of resuscitation is just as important as the correct cryopreservation method. Remember the following points: When removing cells from a liquid nitrogen tank, there may be a cryotube In the case of rupture, it is necessary to use a protective mask and protective clothing. (It is very bad to find a biological injection in this aspect.) Transfer the frozen tube from liquid nitrogen to a hot (high temperature) container, which should be in liquid nitrogen. Complete (?) should quickly dissolve the frozen cells in a 37 ° C water bath and ensure that the cryotube lid is above the water surface to avoid contamination. When the smallest ice cubes are dissolved, the frozen tubes are wiped with 70% alcohol and quickly transferred to a new pre-warmed medium. Cell growth morphology and growth ratio were observed. In fact, cell resuscitation is only a simple experiment, but there are inevitably some details that need attention, otherwise it will not be satisfactory. For example, in terms of personal health: you must remember to do antifreeze work, wear goggles; try to reduce the damage of DMSO to cells.
