Quality of standard ELISA kit depends on which two points - Database & Sql Blog Articles

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Why does standard fetal bovine serum require more care, its quality depends on those two factors, below

Let's

Detailed introduction

:

First, the use of standard fetal bovine serum requires more care

blood

Qing is currently mainly used for cell culture. In this process, the most important thing is to ensure that the culture is not contaminated, and sometimes it needs a certain inactivation treatment. Many people have different opinions on the problem of inactivating fetal calf serum. Experts believe that the elisa kit is prone to bacterial residues in the production process, which can easily cause contamination of the culture samples. When the fetal bovine serum is inactivated, it is easy to kill the nutrients in the serum, but instead There is no benefit in the experiment. As long as the products of the quality-trusted manufacturers are selected, the inactivated fetal bovine serum is also very good.

The serum thus treated has only a slight promotion to the growth of the cells, or has no effect at all, and even the high temperature treatment usually affects the quality of the serum, resulting in a decrease in the cell growth rate. The heat-treated serum, the formation of precipitates will increase significantly, these precipitates observed under an inverted microscope, such as "small black spots", often let researchers mistakenly believe that serum is contaminated, and put serum in 37 In the °C environment, this precipitate will be increased, which makes the researchers mistakenly believe that it is the splitting and amplification of microorganisms.

second,

Standard fetal bovine serum traits:

fetal

A type of bovine serum that refers to serum prepared from blood taken within 2 hours of calf birth. Light yellow, clear transparent liquid

third,

Uses: biochemical research.

For cell tissue culture and the like. The ELISA kit is more complex than the newborn bovine serum, with high protein content and various growth factors, which is more conducive to cell growth and reproduction.

fourth,

The quality of the ELISA kit depends on these two aspects.

1. The object of the material.

2. The process of taking materials.

The fetal calf serum used for the fetal calf should be healthy and disease-free. Within the specified number of birth days, the extraction process should be carried out in strict accordance with the operating procedures, and the prepared serum should undergo strict quality identification. Special requirements are:

a. Filter through the filter to ensure sterilization;

b. Prove that the serum used does not contain inhibitors of the vaccine virus produced;

c. Free of contamination by bacteria, mold, mycoplasma and viruses, some countries require no bacteriophage contamination;

d. have good support for the reproduction of cells;

e. Must be from a herd or country that has documented no bovine spongiform encephalopathy. And should have an appropriate monitoring system.

Hengyuan Immunology Third-party testing center provides you with high-quality fetal bovine serum, and represents the high-quality serum of major brands such as Gibco, Sigma, Hyclone, etc. Welcome to consult!