What! Does the ELISA kit wash even have patterns? -Huaqiang Electronic Network

EL-C1600N100013-B

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In an experiment, it can be done in a variety of ways. Like an item, it can be used for a variety of purposes as long as you use your brain. This step of washing is an indispensable part of the ELISA experiment. It is also a step in this step. Do you want to know which methods? Shanghai Jinma introduces you to two different patterns of washing in an ELISA experiment.

Pattern first-class water rinse

This was originally used for the washing of the bead carrier, which was only distilled water or even tap water. A special device is attached during washing to continuously roll and wash the beads under the impact of running water. After rinsing for 2 minutes, the liquid is absorbed, and then immersed in distilled water for 2 minutes, and dried.

Technician introduction: This method is more thorough and easy and fast. It has been shown in experiments that the flow rinse method is also suitable for the washing of microtiter plates. Try to increase the water flow or increase the water pressure during washing, so that the water flow impacts the surface of the plate hole, and the washing effect is better.

Pattern two soak

1. Drain or dry the reaction solution in the well;

2. Wash it once with the washing solution (after filling the plate with the washing liquid, it will be removed);

3, soaking, the washing liquid is filled into the plate hole, placed for 1-2 minutes, intermittent shaking, soaking time can not be shortened arbitrarily;

4. Drain the liquid in the hole. Drying should be thorough, pumping with a water pump or vacuum pump, or squirting the liquid on a clean towel or absorbent paper after removing the liquid;

5. Repeat operations c and d for 3-4 times (or as specified). In the indirect method, if the background is higher, the number of washings or the soaking time can be increased.

The above content is the two different patterns washed in the ELISA experiment. If you have other content that you don't understand, you can leave a message on this website. You can also directly call our company's business staff. We will arrange the professional as soon as possible. The technical staff will answer you.