Foreword
At present, there is no method for determining the starch content in corn germ buds in China. The method for measuring other starches is cumbersome, time consuming, laborious, and uneconomical, which brings inconvenience to the test. New generation
Polarimeter
It can solve the measurement time of the saver. Current starch sugar producers commonly use refractometers or drying methods to determine the solids content of starch syrup. The refracting method measures the solid content based on a simple functional relationship between the refractive index of the syrup and its moisture content, while the drying method directly evaporates the water in the syrup without being affected by the characteristics of the sample. However, the drying method is time consuming and complicated. Many scholars in China have made different experiments through experiments.
DE
The relationship between the value and the difference between the refracting method and the drying method, and proves that the correlation is very good. Measuring the soluble solid content (dry matter content) by refractive method can greatly improve the efficiency of product testing, reduce production costs, and increase economic benefits.
1 Scope of application
The method is suitable for the determination of starch content in feed, and is also applicable to the determination of starch content in other cereals and products thereof.
2 method summary
Polarimeter
Measure the optical rotation and multiply by a certain coefficient to determine the starch content.
. 3. Instruments and reagents
Fully automatic
Polarimeter
One,
Scaled
1.124%
hydrochloric acid
,
30%
Zinc sulfate solution
4. Operation steps
Ready to weigh
2.50
Gram smashed sample
50ml
Colorimetric tube, plus
25ml 1.124%
Hydrochloric acid, shake well. Put the colorimetric tube into boiling water and cook
15
After a minute, it was placed in cold water and cooled to room temperature. Sulfuric acid mirror
5ml,
Dilute to the mark with distilled water.
If you measure the content of other cereal starches such as corn and rice, use
5%Uranyl acetate
,Use
5% Uranyl acetate
5. Calculation method
In the formula: α: optical rotation
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