The most commonly used method for antigens - ELISA test kits

The most common method of antigen-ELISA test kit ELISA test kit excess antigen and solid phase antibody and enzyme-labeled antibody respectively, ELISA detection kit antigen most commonly used method and no longer form a sandwich complex, the results will be lower than the actual content. A false negative result can even occur when the hook effect is severe. A mixed solution of the sample to be tested and a certain amount of the enzyme-labeled antigen is added to the tube to be reacted with the solid phase antibody. If there is no antigen in the sample to be tested, the enzyme-labeled antigen can smoothly bind to the solid phase antibody.
If the sample contains an antigen, it binds to the solid phase antibody at the same opportunity as the enzyme-labeled antigen, competitively occupies the opportunity to bind the enzyme-labeled antigen to the solid-phase carrier, and combines the enzyme-labeled antigen with the solid-phase carrier. The amount is reduced. Only the enzyme-labeled antigen is added to the reference tube, and after incubation, the binding of the enzyme-labeled antigen to the solid-phase antibody can reach the most sufficient amount. washing.
ELISA test kit plus substrate color development: the reference tube has the highest color due to the combination of the enzyme-labeled antigen. The difference between the reference tube color depth and the color depth of the tube to be tested represents the amount of the sample antigen to be examined. The lighter the color of the tube to be tested, the more antigen content in the specimen. This enzyme-labeled antigen or antibody retains both its immunological activity and the activity of the enzyme.
The ELISA test kit measures the antigen or antibody on the surface of the solid phase carrier in different steps in the assay, the test sample (the antibody or antigen assayed therein) and the enzyme target antigen or antibody. The antigen-antibody complex formed on the solid phase carrier is separated from other substances by washing, and finally the amount of the enzyme bound to the solid phase carrier is proportional to the amount of the test substance in the sample. After adding the substrate of the enzyme reaction, the substrate is catalyzed by the enzyme to become a colored product, and the amount of the product is directly related to the amount of the test substance in the sample, and the ELISA test kit