Micro-immunoelectrophoresis experimental steps - Database & Sql Blog Articles

1. Spread the agar plate as shown in the figure below, and then punch the groove as shown below. Do not pick out the agar in the groove during electrophoresis (the two longitudinal parallel sides of the groove are not scratched). 2. Load the top of the agar plate with the antigen solution filled with the indicator protein. 3. Electrophoresis is the same as above, and the current intensity is 2~4 mA/cm. Electrophoresis can be stopped when the protein is indicated to reach the edge of the filter paper. It usually takes 40 to 60 minutes. 4. After the diffusion electrophoresis is finished, pick up the agar gel with a toothpick from the groove in the middle of the agar plate and add the immune serum. Place the agar plate in a temperature box (or in a Petri dish), cover the lid and leave it overnight. The sedimentation line appearing inside the gel can be observed the next day. In general, each precipitation line represents an antigenic component. The purity of the antigen and the type of the antigen protein can be judged based on the number and position of the precipitate. Microimmunoelectrophoresis is commonly used to analyze the serum components of a sample. 5. Observing the immunoelectrophoresis map can directly observe the antigen-antibody precipitation line, or it can be observed after staining with 0.1% amino black. If you need to save, you can take a photo.